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细菌降解非离子表面活性剂的研究
摘要点击 647  全文点击 1062  投稿时间:1996-05-15  
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中文关键词  假单胞杆菌52(pseudomonos 52)  Weeksella 6  非离子表活剂AEO-9  SA-20  生物降解
英文关键词  Pseudomonos sp 52  Weeksella sp 6  nonionic surfactant  biodegradation
作者单位
林力 中国科学院微生物研究所 北京 100080 
杨惠芳 中国科学院微生物研究所 北京 100080 
夏星辉 北京师范大学环境科学研究所 北京 100875 
许嘉琳 北京师范大学环境科学研究所 北京 100875 
中文摘要
      从石油污染的土壤中分离到2株能利用非离子表面活性剂AEO-9和SA-20作为唯一碳源生长的细菌。这2株窗被定名为假单胞杆菌52(Pseudomonos 52)和Weeksella 6.最适于这2株菌利用非离子表活剂AEO-9的培养条件是乙酸胺作为氮源,PH7,30℃;添加少量萄糖能促进表活剂的降解。降解速率的研究表明,当AEO-9的初始浓度为5000mg/L时,52号菌和6号菌都能在2周内将其去除85%,并在4周内将其去除95%.52号菌的休眠细胞能在PH7,30℃和细胞浓度(湿重)200mg/ml的条件下,在6.5h内将同样初始浓度的AEO-9降解60%.
英文摘要
      Two strains isolated from soil contaminated by petroleum chemicals,Pseudomonos sp.strain 52 and Weeksella sp.strain 6 could utilize nonionic sndactants(AEO-9 and SA-20)as sole source of carbon for growth.The experiment results showed that the optimum source of nitrogen was ammonium acetate,the optimum PH and temperature for the degradation were 7.0 and 30℃.It was found that addition of glucose enhanced biodegradation of AEO-9.With the initial concentration of 5 000 mg/L the growth cells of the two strains had an AEO-9 removal efficiency of 85% and 95% within 2 weeks or 4 weeks,respectively. The biodegradation rate of AEO-9, in the same initial concentration,by resting cells of strain 52was 60% within 6.5 hours under the optimum degradation condition in which PH,temperature and cell density (wet weight/volume)was 7.0、30C and 20%,respectively.

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