| 简易、快速检测有机磷农药的酶片和生色基质片 |
| 摘要点击 693 全文点击 646 投稿时间：1994-09-30 |
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| 中文关键词 有机磷农药 检测 酶片 生色基质片 |
| 英文关键词 organophosphorus pesticides detection enzyme ticket substrate ticket |
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| 中文摘要 |
| 研制出简易、快速检测有机磷农药的酶片和生色基质片。用胆碱酯酶抑制试验检测水中的有机磷农药,检测灵敏度在0.01—10mg/L范围,适宜温度在25—35℃之间,分析周期约为15—20min。甲胺磷和水胺硫磷等硫代磷酸酯类农药经溴水活化后检测灵敏度也达上述水平。实际水样检测表明,检样中农药含量在检测灵敏度范围内时,阳性结果相当明显,溶液吸光度比值A对照/A检样>2。由于检测时无需配制试剂和使用仪器,本法特别适宜于现场检测。 |
| 英文摘要 |
| The cholinesterase inhibition test made it possible to detect organophosphorus pesticides (OPs) compounds in water at 25—35℃ in 15-20 min, and the sensitivity of the method was in the range of 0.01—10 mg/L. Most thiophosphorus pesticides, such as methamidophos and optunal, can also be detected in those levels after they are oxiding with bromine water. This enzyme inhibition assay has ben applied to the detection of pesticide in natural waters, and it was found that the positive results were quite obvious as soon as the concentration of OPs were not lower than the detection limits. The method is particularly suitable for field dtection because there is no need for expensive instruments and preparation of reagents. |
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